Description
Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofSEMAPHORIN-3A in the sample, This SEMAPHORIN-3A ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusSEMAPHORIN-3A concentration. The concentration ofSEMAPHORIN-3A in the samples is then determined by comparing the O.D. of the samples to the standard curve